NBCPBH

Production of new bioactive compounds by plants and bacteria using new and improved halogenases
Acronym: NBCPBH

Project coordinator
- Prof. Karl-Heinz van Pee - TU Dresden - Germany

Project leaders
- Prof. Sarah O'Connor - University of East Anglia/John Innes Centre, Norwich - UK
- Dr. Rebecca Goss - University of St. Andrews - UK
- Prof. Jutta Ludwig-Müller - TU Dresden - Germany
- Dr. Alfredo Aires - Universidade de Trás-os-Montes e Alto Douro, Vila Real - Portugal
- Dr. Anika Schumann - Vita 34 AG, Leipzig - Germany

The in vitro use of halogenases is hampered by a fast inactivation of the enzymes under reaction conditions. To allow the use of halogenases in biotechnological processes, the reason for inactivation will be elucidated and stabel halogenase variants will be constructed. Since their application is also restricted by the high substrate specificity variants with a relaxed substrate specificity will be constructed. Already available tryptophan halogenases and variants from the project will be used to transform plants and bacteria producing tryptophan-containing secondary metabolites or indole derivatives to obtain new halogenated compounds produced in vivo. Using mild chemistries, the halogen atoms can be exchanged against different functional groups leading to novel compounds. Cultivation of transformed plants producing novel halogenated compounds will be optimised, the metabolic profile analysed and the biological and antioxidant activities of the compounds will be investigated.