IMAPPROT

Integrated, multi-host approach for the improved microbial production of high quality therapeutic enzymes and proteins 

Acronym: IMAPPROT

Project coordinator

- Prof. Antonio Villaverde – Universitat Autonoma de Barcelona - Spain

Project leaders

- Dr. Monika Bollok – Bioingenium – Spain

- Dr. Simo Schwartz – Vall d’Hebron University Hospital – Spain

- Dr. Saloheimo Markku – VTT Technical Research Centre – Finland

- Ms Lisa Tutino – University of Naples Federico II – Italy

- Prof. Diethard Mattanovich – University of Natural Resources and Applied Life Sciences – Austria

Abstract

Many enzymes and other proteins of pharmacological interest and addressed to human therapy are presently produced by cultured mammalian cells through expensive procedures, resulting in high-priced products. The choice of mammalian cells instead of simpler microbial systems is often prompted by the occurrence of structural properties (disulfide bridge, proper conformation, glycosylation etc) linked to the requested biological activity, that cannot be fully reproduced in microbial cells.  However, the incorporation of new hosts for therapeutic protein production and the fast-moving advances in protein and metabolic engineering, strain and process design and physiological analysis of stress responses make the production of complex therapeutic proteins in microbial hosts a more feasible and economically competitive industrial biotechnology concept.

The objective of this proposal is to obtain functional therapeutic proteins by microbial production, and to create scientific data with wide applicability to diverse protein production problems in industrial biotechnology. The project will combine modular protein engineering approaches with metabolic and process engineering to obtain functional enzymes through microbial production processes, and will comparatively analyze the suitability of alternative microbial hosts, either conventional or novel, regarding the biological properties of a target therapeutic protein, the human alfa‑galactosidase A.

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